Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)

Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital essential aliphatic amines which also present in plants. Although ethylene polyamines involved fruit ripening, genes coding them must take part other biosynthetic pathways. In play an important role development of salt stress tolerance, responses for biotic abiotic stresses. Exogenous application all three main (Put, Spd, Spm) increase tolerance plants, but, accordingly to previous experiments, has effect on enhancement tolerance. Nicotiana tabacum L. plants were grown vitro MS medium, treatments follows: arginine (150 mg l-1), (10 l-1). Proline, chlorophyll a, b polyamine contents measured. The obtained results show that decarboxylase synthase metabolism, cannot be enhanced by exogenous addition precursor molecules. On contrary, gene a positive lower-class forms polyamines.